RESUMO
INTRODUCTION: The use of peripheral hematopoietic progenitor cells (HPCs) is the cell choice in autologous transplantation. The classic dose of granulocyte-colony stimulating factor (G-CSF) for mobilization is a single daily dose of 10µg/kg of patient body weight. There is a theory that higher doses of granulocyte-colony stimulating factor applied twice daily could increase the number of CD34(+) cells collected in fewer leukapheresis procedures. OBJECTIVE: The aim of this study was to compare a fractionated dose of 15µg G-CSF/kg of body weight and the conventional dose of granulocyte-colony stimulating factor in respect to the number of leukapheresis procedures required to achieve a minimum collection of 3×10(6) CD34(+) cells/kg body weight. METHODS: Patients were divided into two groups: Group 10 - patients who received a single daily dose of 10µg G-CSF/kg body weight and Group 15 - patients who received a fractioned dose of 15µg G-CSF/kg body weight daily. The leukapheresis procedure was carried out in an automated cell separator. The autologous transplantation was carried out when a minimum number of 3×10(6) CD34(+) cells/kg body weight was achieved. RESULTS: Group 10 comprised 39 patients and Group 15 comprised 26 patients. A total of 146 apheresis procedures were performed: 110 (75.3%) for Group 10 and 36 (24.7%) for Group 15. For Group 10, a median of three (range: 1-7) leukapheresis procedures and a mean of 8.89×10(6) CD34(+) cells/kg body weight (±9.59) were collected whereas for Group 15 the corresponding values were one (range: 1-3) and 5.29×10(6) cells/kg body weight (±4.95). A statistically significant difference was found in relation to the number of apheresis procedures (p-value <0.0001). CONCLUSIONS: To collect a minimum target of 3×10(6) CD34(+) cells/kg body weight, the administration of a fractionated dose of 15µg G-CSF/kg body weight significantly decreased the number of leukapheresis procedures performed.
RESUMO
The use of peripheral hematopoietic progenitor cells (HPCs) is the cell choice in autologous transplantation. The classic dose of granulocyte-colony stimulating factor (G- CSF) for mobilization is a single daily dose of 10 µg/kg of patient body weight. There is a theory that higher doses of granulocyte-colony stimulating factor applied twice daily could increase the number of CD34+ cells collected in fewer leukapheresis procedures. Objective: The aim of this study was to compare a fractionated dose of 15 µg G-CSF/kg of body weight and the conventional dose of granulocyte-colony stimulating factor in respect to the number of leukapheresis procedures required to achieve a minimum collection of 3 × 106 CD34+ cells/kg body weight. Methods: Patients were divided into two groups: Group 10 - patients who received a single daily dose of 10 µg G-CSF/kg body weight and Group 15 - patients who received a fractioned dose of 15 µg G-CSF/kg body weight daily. The leukapheresis procedure was carried out in an automated cell separator. The autologous transplantation was carried out when a minimum number of 3 × 106 CD34+ cells/kg body weight was achieved. Results: Group 10 comprised 39 patients and Group 15 comprised 26 patients. A total of 146 apheresis procedures were performed: 110 (75.3%) for Group 10 and 36 (24.7%) for Group 15. For Group 10, a median of three (range: 1-7) leukapheresis procedures and a mean of 8.89 × 106 CD34+ cells/kg body weight (±9.59) were collected whereas for Group 15 the corresponding values were one (range: 1-3) and 5.29 × 106 cells/kg body weight (±4.95). A statistically significant difference was found in relation to the number of apheresis procedures (p-value <0.0001). Conclusions: To collect a minimum target of 3 × 106 CD34+ cells/kg body weight, the administration of a fractionated dose of 15 µg G-CSF/kg body weight significantly decreased the number of leukapheresis procedures performed.
Assuntos
Humanos , Masculino , Feminino , Recém-Nascido , Lactente , Pré-Escolar , Criança , Adolescente , Adulto Jovem , Fator Estimulador de Colônias de Granulócitos , Mobilização de Células-Tronco Hematopoéticas , Transplante de Células-Tronco Hematopoéticas , Leucaférese , Transplante de Células-Tronco de Sangue Periférico , Transplante AutólogoRESUMO
Os autores apresentam um raro caso de micobacteriose atípica bem como complicaçöes imunológicas, em um paciente portador de LMC fase crônica submetido a um TMO alogênico com célula-tronco periférica de doador HLA idêntico. Säo discutidas as características clínicas do paciente ao diagnóstico, a terapêutica instituída e as possíveis causas de óbito
Assuntos
Humanos , Masculino , Adulto , Infecções por Mycobacterium não Tuberculosas/diagnóstico , Infecções por Mycobacterium não Tuberculosas/etiologia , Transplante de Medula Óssea/efeitos adversosRESUMO
Relatam um caso clínico de esferocitoses hereditária no período neonatal, bem como uma revisäo de literatura. Comentam os aspectos clínicos, laboratoriais, evolutivos e terapêuticos. Destacam, ainda, a importância do diagnóstico diferencial com a doença hemolítica por incompatibilidade ABO.
Assuntos
Humanos , Masculino , Recém-Nascido , Esferocitose Hereditária/diagnóstico , Diagnóstico DiferencialRESUMO
Relatam um caso clinico de esferocitose hereditaria no periodo neonatal, bem como uma revisao de literatura. Comentam os aspectos clinicos, laboratoriais, evolutivos e terapeuticos. Destacam, ainda, a importancia do diagnostico diferencial com a doenca hemolitica por incompatibilidade ABO.